Egyptian propolis was analyzed by gas chromatography mass spectrometry.
Meaning of "Propolis" in the German dictionary
Seven compounds were identified in Egyptian propolis for the first time. The constituents were phenolic acid esters It was clear that phenolic acid esters are present in a major quantity Evaluation of Egyptian propolis as immunostimulant, antiviral, antibacterial and antifungal agent were done and showed that the Egyptian propolis has such activities. Propolis samples from Albania, Austria, Bulgaria, France, Egypt, Germany and Mongolia have been investigated and the results obtained compared with propolis from England.
The quantitative differences obtained could be due to the participation of different poplar species. In some regions small amount of propolis could be collected from other plants, too. These esters have not been found till now in propolis. A series of triterpenes in Egyptian propolis, lanosterol were also identified. Phenylethyl-trans-caffeate, pinokansin acetate and galangin were predominant in German propolis. Pinocembrin was predominant in Bulgarian and British propolis and trans-p-coumaric acid was in German and British. The antimicrobial activity of propolis collected from different countries against Staphylococcus aureus ; Escherichia coli , and Candida albicans were investigated.
All propolis samples showed an inhibition in the growth of all examined microorganisms but the inhibition varied according to the propolis origin. It was obvious that German and Egyptian propolis showed the highest antimicrobial activity against Staphylococcus aureus while it was highest in Mongolian, German and Egyptian propolis against Escherichia coli , but Egyptian and Austrian propolis has the highest activity against Candida albicans. Propolis, a natural resin produced by honey bees, that displays strong antimicrobial activity, has been used as a chemotherapeutic agent since ancient times.
The anti-protozoan properties of different propolis extracts were studied regarding T. Ethanolic EEP and dimethylsulphoxide extracts DEP were both active against the three forms of the parasite, with the former being more active than the latter against the vertebrate forms, amastigotes and trypomastigotes.
The effect was found to be temperature dependent. Treatment of infected peritoneal macrophages and heart muscle cells with EEP strongly inhibited infection levels. The utilization of propolis as a possible antitrypanosomal agent is discussed. Propolis, the resinous hive product collected by bees, is important in the defense of the hive.
The effects of propolis on growth and glucosyltransferase activity of Streptococcus sobrinus , Streptococcus mutans PS14 and Streptococcus cricetus OMZ61 in vitro, and on dental caries in rats infected with S. Propolis had antimicrobial activity against S. In rats inoculated with S. Dental caries was markedly decreased by the multiple actions of propolis which had antimicrobial activity, inhibited water-insoluble glucan synthesis, and inhibited glucosyltransferase activity.
No toxic effects of propolis on the growth of rats were observed under experimental conditions in this study. These results suggest that propolis can control dental caries in the rat model system. Abstract The effect of a water-soluble derivative WSD of propolis on the classical pathway CP and the alternative AP complement activity has been investigated. The in vitro experiments show that WSD inhibits both pathways and the effect depends on the source of complement.
The suppression of complement-mediated haemolysis proves to be time- and temperature-related. High WSD concentrations cause direct damage of the target erythrocytes. The estimation of C3-residual activity indicates that the preparation diminishes C3 functional activity.
The alteration of serum alternative pathway AP complement level was observed. The WSD also influenced the process of acute inflammation provoked by zymosan in mice.
The effect was strongly dependent on the route of WSD administration. Caffeic acid phenethyl ester CAPE is a phenolic antioxidant derived from the propolis of honeybee hives. CAPE was shown to inhibit the formation of intracellular hydrogen peroxide and oxidized bases in DNA of O-tetradecanoylphorbolacetate TPA -treated HeLa cells and was also found to induce a redox change that correlated with differential growth effects in transformed cells but not the nontumorigenic parental ones.
Mediated via the electrophile or human antioxidant response element hARE , induction of the expression of NAD P H quinone oxidoreductase NQO1 and glutathione S-transferase Ya subunit genes by certain phenolic antioxidants has been correlated with the chemopreventive properties of these agents.
Here, we determined by Northern analysis that CAPE treatment of hepatoma cells stimulates NQO1 gene expression in cultured human hepatoma cells HepG2 , and we characterized the effects of CAPE treatment on the expression of a reporter gene either containing or lacking the hARE or carrying a mutant version of this element in rodent hepatoma Hepa-1 transfectants. A dose-dependent transactivation of human hARE-mediated chloramphenicol acetyltransferase cat gene expression was observed upon treatments of the Hepa-1 transfectants with TPA, a known inducer, as well as with CAPE.
The combined treatments resulted in an apparent additive stimulation of the reporter expression. To learn whether this activation of cat gene expression was effected by protein kinase C in CAPE-treated cells, a comparison was made of cat gene activity after addition of calphostin, a protein kinase C inhibitor. Calphostin reduced the cat gene induction by TPA but not by CAPE, suggesting that stimulation of gene expression in this system by these agents proceeds via distinct mechanisms. Band-shift experiments to examine binding of transactivator proteins from nuclear extracts of treated and untreated cells to a hARE DNA probe showed that TPA exposure increased the binding level.
In contrast, binding of factors to this probe was inhibited after either in vivo treatment of cells with CAPE or in vitro addition of this compound to the nuclear extract. The action of ethanol extract of propolis EEP on laboratory animals. In the experiment on rats the ethanol extract of propolis EEP injections caused an activation of all experimental enzymes. Abstract Propolis is a natural product produced by the honey bee.
The extract contains amino acids, flavanoids, terpenes and cinnamic acid derivatives. In various in vitro models propolis extract was shown to inhibit platelet aggregation and to inhibit eicosanoid synthesis, suggesting that it might have potent antiinflammatory properties. In both models, the extract showed potent dose-related antiinflammatory activity, which compared well with that of diclofenac as a reference standard. The extract was then tested on an isolated sensitized guinea pig lung preparation to study its effect on the release of prostaglandins, leukotrienes and histamine.
It is concluded that propolis extract has potent antiinflammatory properties in vivo. Its activity can be well correlated with its effects on the release of various mediators of inflammation. Kirienko A. Tcherkasova L. Zaharteva V. Gladtchun V. Klevanik L. Clinical and x-ray examinations have demonstrated a high efficacy of such technique in acute, exacerbated, and chronic forms of periodontitis.
This filler is characterized by an anesthetizing effect; it resolves behind the root canal apex within months, is preserved in the root canals, does not stain the tooth crown, promotes regeneration of the bone structures, and prolongs the effect of 0. El profesor viene estudiando la actividad del propoleo desde Propolis designates a mixture of gums, resins and balms, of viscous consistency, which are gathered on certain parts buds and bark, mainly of vegetables especially coniferous trees by honeybees. They bring this back to the hive, where it is modified and mixed with other substances essentially their own wax and salivary secretions.
In this study, the hepatoprotective and therapeutic effects of propolis ethanol extract on chronic alcohol-induced liver injuries were investigated in rats. After sacrifice, serum transaminases GOT, GPT , triacylglyceride and hepatic triacylglyceride HTG concentration were assayed to observe liver injuries induced by chronic alcohol abuse. In addition, the phenomenon of alcohol induced fatty liver were also observed by histopathological changes. Different doses of propolis ethanol extract were p. Three syndromes of dissociated hypotension of the CSF were described: 1 at the level of the Magendie-Luschka openings; 2 at the level of the aqueduct of Sylvius; 3 at the level of the third ventricle.
Each level of hypotension of the CSF was attended by a specific clinical syndrome. The parameters of the progressive course of the disease made it possible upon their identification to begin the rehabilitation of patients at the earliest stage which led to a faster compensation. Special attention was given to apitherapy , phytococtails and instillations of special coctails for controlling the dissociation between CSF hypotension and hydrocephaly. The conducted rehabilitation made it possible to significantly reduce the incidence of crises indicative of the decompensation of brain injury. Positive allergic reactions to propolis were observed in 25 patients 4.
In view of the relatively high incidence of allergic reactions and the appearance of pseudo-cross-sensitivity to another common allergen, balsam of Peru, propolis should not be used in topical medicaments or as a component of cosmetic preparations. The purpose of this study was to examine histologically the effects of propolis topical application to dental sockets and skin wounds.
The rats were sacrificed at 3, 6, 9, 15 and 21 days after the operation. The specimens were subjected to routine laboratory studies after staining with hematoxylin and eosin. It was concluded that topical application of propolis hydro-alcoholic solution accelerated epithelial repair after tooth extraction but had no effect on socket wound healing.
Abstract A study was conducted to analyze the effects of propolis mouth rinse on the repair of surgical wounds after sulcoplasty by the modified Kazanjian technique. The patients returned 7, 14, 30, and 45 days after surgery for cytological and clinical evaluation. It was concluded that: 1 the mouth rinse containing propolis in aqueous alcohol solution aids repair of intra-buccal surgical wounds and exerts a small pain-killing and anti-inflammatory effect; 2 the vehicle employed has a minor irritant effect on intra-buccal surgical wounds; 3 exfoliative cytology allows epithelization of intrabuccal surgical wounds.
The protective effect of honeybee aqueous propolis extract APE against the hepatotoxicity of carbon tetrachloride was investigated using isolated liver-cell suspensions as the experimental model. Various concentrations of the extract were preincubated with the hepatocyte suspensions for 30 min before being subjected to the hepatotoxin for a further 30 min. The hepatocyte toxicity was assessed using three parameters, namely, the release of lactate dehydrogenase, the formation of lipid peroxides and the depletion of intracellular reduced glutathione.
It was found that a dose-related protection against the induced cell injury was conferred by APE as evidenced by its inhibitory influence on the changes induced by CCl4 on the measured parameters. The hepatocyte protective effect of APE is probably a result of its antioxidant and free-radical-scavenging properties which in turn help to maintain the intracellular level of reduced glutathione. There was studied the therapeutic efficiency of ocular medical propolis films OMF in 35 patients with postherpetic trophic keratitis and in 20 with postherpetic nebula. OMF were applied behind the lower eyelid at bedtime during days.
All the patients endured the propolis films well. OMF accelerated the cornea epithelization. Epitheliopathy and micropoint edema of cornea epithelium rapidly disappeared. Time of patients recovery reduced nearly twice P, in comparison with the control group—from On the average their visual acuity increased in two times—from 0. Since many years ago are known curative effects of propolis on different lesions due, mainly, to more than 30 biologically active elements isolated from it. It is a wordly accepted opinion that propolis is one of the most useful substances elaborated by bees.
Despite it, its use in our country is relatively recent. Several investigators, specially in Matanzas, study curative effects of propolis in the field of human and animal medicine. In other countries it has been used in stomalogy, but we have no knowledge about its therapeutical effects in the periodontal disease or its effectiveness in oral ulcers.
Therefore, a therapeutical form has been elaborated, which is provisionally called Propolan, for the treatment of two entities: chronic gingivitis and stomatitis of different etiology. The clinical cases supporting effectiveness found with the treatment applied in our setting are presented.
Apitherapy venom of bees and apiculture products was included into combined treatment of 93 in-patients with pulmonary tuberculosis. Apitherapy had a beneficial effect on the organism of tuberculosis patients, manifested by enhancement of the treatment effectiveness and normalization of indices of endocrine system.
It is recommended that the instruction on apitoxinotherapy be amended, in particular, by substantially supplementing the paragraph with indications and contraindications for giving it in active tuberculosis. The authors suggest that apitherapy should be used in the treatment of patients with chronic non-specific pulmonary diseases ChNPD in order that it might be more effective.
Apitherapeutic complex bee venom and bee keeping apiculture produce has been applied to the treatment of ChNPD patients. High effectiveness of apitherapy in a combined treatment of ChNPD patients was demonstrated as was their stimulating and normalizing influence on the function of the adrenals. In the first seminar, two Brazilian professors and two Japanese gave talks on production, nature and application of propolis in past and in future.
Disconnection among researchers, producers, and consumers was pointed and future activities of the association was promised to offset the gap. Consumption of propolis in Japan has increased rapidly during a decade, paralleling with the advances of research on propolis. Japan Propolis Association, composed of ca. Collaboration will be needed throughout the world, especially among the researchers themselves and organizations such as Apimondia and other domestic associations in the concerned countries.
The compound showed anti-tumor activity more effective than that of 5-fluorouracil to transplantable human tumor cell lines when tested on histoculture drug response assay system. Additionally, alanine aminotransferase ALT in serum and liver triglycerides were determined in all animals. The morphometric study included the count of ballooned cells at the zone III of the Rappaport acini and the assessment of a software program to estimate the extension of steatosis area. A significant reduction of ballooned cells count in liver was observed at three dose levels of RP extract with respect to rats treated only with CCl4.
Also, a certain reduction of steatosis degree as well as decreased concentration of liver triglycerides and ALT activity were found in three groups of rats treated with RP extract and CCl4 in relation to those treated with the hepatotoxin. Taken together, the histopathological and biochemical findings show hepatoprotective effects of RP extract in CCl4-induced liver damage in rats, probably due to the antioxidant effect of RP.
Pinocembrin 5,7-dihydroxy-flavanone —a component of the bee product propolis—was tested for its in vivo activity against Candida albicans in mice. It was shown that the intravenous infection of AB-Jena mice with 2. The question of effectiveness of pinocembrin in vivo should be cleared up in further pharmacokinetic investigations.
After a survey of the literature on the antimicrobial activity of the bee product propolis, the authors discuss their own findings as compared to the chemotherapeutical agents streptomycin, oxytetracycline, chloramphenicol, nystatin, griseofulvin and sulphamerazine. According to the results obtained by testing 25 isolated constituents on Bacillus subtilis , Staphylococcus aureus , Candida albicans and Trichophyton mentagrophytes , the antimicrobial properties of this mixture of natural substances are mainly attributable to the flavonoids pinocembrin, galangin, pinobanksin, pinobanksinacetate as well as to the p-coumaric acid benzyl ester and a caffeic acid ester mixture.
None of the isolated substances was as potent as the antibiotics tested for the purpose of comparison. The relatively good antimycotic activity of the 5,7-dihydroxyflavanone pinocembrin seems noteworthy. Finally, possible mechanisms of the antimicrobial action of the flavonoids are discussed. The ethanol extract of propolis suppressed prostaglandin and leukotriene generation by murine peritoneal macrophages in vitro and during zymosan- induced acute peritoneal inflammation in vivo.
Dietary propolis significantly suppressed the lipoxygenase pathway of arachidonic acid metabolism during inflammation in vivo. CAPE was the most potent modulator of the arachidonic acid cascade among the propolis components examined. The effect of the natural bee product propolis on the physiology of microorganisms was investigated using B.
An ethanolic extract of propolis had a bactericidal effect caused by the presence of very active, but labile, ingredients. The exact bactericidal effect of propolis was species dependent: it was effective against gram-positive and some gram-negative bacteria. Propolis and some of its cinnamic and flavonoid components were found to uncouple the energy transducing cytoplasmic membrane and to inhibit bacterial motility. These effects on the bioenergetic status of the membrane may contribute to the antimicrobial action of propolis and its observed synergism with selected antibiotics.
The method for an exact diagnosis in children was duodenal aspiration and in adults duodenal mucosa frotis by means of duodenoscopy. Similar studies were carried out as a cure criterium in a 5-day term after being through with the treatment. This work shows the success of this natural product, which is very easy to obtain in Cuba and with no side effects in the treatment of this intestinal parasitism, what is of great economical importance for our countries.
The results of a study to investigate the effectiveness of a propolis-containing mouthrinse in the inhibition of de novo plaque formation are presented. Subjects used a propolis-containing rinse, a negative control and a positive control in a double-blind, parallel, de novo plaque formation study design. The chlorhexidine mouthrinse was significantly better than the others in plaque inhibition.
The propolis- containing rinse was marginally better than the negative control, but this difference was not significant. Seit 5 Jahren unternehmen die Verfasser empirische und Laboruntersuchungen mit Propolis aus allen Regionen Brasiliens. Email: niraldo unisul. Propolis is a natural product produced by bees. In the folk medicine propolis is used in the treatment of respiratory diseases. The objective this work is to evaluate the effect of ethanolic extract of propolis EEP on the smooth muscle of guinea pig isolated trachea, and to investigate its pharmacological mechanisms of action.
Guinea pigs both sexes, g were killed by cervical dislocation, trachea was removed and cut in six transversal segments containing three rings. The isometric relaxant responses were measure by means of force displacement transducer TRI coupled to a Polygraph Letica Scientific Instruments. Incubation of the phenolic compounds isolated from propolis, in the bath, produced a relaxant response in the guinea pig isolated trachea.
The EEP 10 -6 g. Presence of KCl 40 or 80mM in the medium produced a inhibition of Apamin 0. Iberiotoxin 0. L-NOArg 10 or mM inhibited Take together these results shown that multiples mechanisms of actions are involved in the relaxant propolis-induced response on the smooth muscle of guinea pig isolated trachea. These results, also indicated that the principal propolis relaxant signaling pathway involved in response include direct activation of potassium channels, modulation of VIP or b-adrenoceptors receptors and the nitric oxide pathway.
Lipid peroxidation was activated in salmonellosis. Content of hydroperoxides and malonic dialdehyde was increased in mice liver tissue. At the same time, the activity of antioxidative enzymes was altered. Continuous administration of propolis stabilized lipid peroxidation, thus suggesting that the substance could be used as a drug in medicine. The constant interest of researchers in new methods of prophylaxis and treatment of gastrointestinal diseases is caused by a high frequency of these diseases and by the absence of effective methods of control.
The application of apitherapeutics in the prophylaxis and treatment of gastrointestinal diseases is based on the anti-inflammatory, analgesic, immunogenous and antioxidant effects of these products. Acute intestinal infections in children are often of the lingering type. They results in disbacteriosis, malabsorption and hypopolyvitaminosis. The destruction of enterocytes, the modification of the normal intestinal flora, the breakdown of the system of general and local immune responses are typical of the intestinal inflammation. Therefore, it is very important to find a drug which is able to normalise the immunity.
Propolis is a product that enhances immunity in children with intestinal infections. To determine the immune response of the organism, we studied the spontaneous migration of leukocytes and the level of serum fibronectin, that is a non-specific opsonin. Thus, we may conclude that propolis improves the defensive capacity of the organism and, therefore, may be prescribed to treat patients with gastroduodenal ulcer.
Besides, propolis may also be used to treat children with intestinal infections. Facultad de Ciencias Medicas. No se observaron cambios durante el tratamiento ni al finalizar el mismo. No se produjeron reacciones adversas. Email: ykpark fea. The respective samples were extracted with ethanol. The ethanolic extracts of propolis EEP were analyzed by physicochemical methods such as appearance of EEP, measurement of absorption spectra by UV-spectrophotometry, reversed phase-thin layer chromatography RP-HPTLC , reversed phase high performance liquid chromatography RP-HPLC and then the EEPs were evaluated their physiological activities such as antioxidant activity, antimicrobial activity, assay of cytotoxic activity to cancer cells, and HIV-growth inhibition assays.
Among 12 groups of propolis, five groups of propolis were collected from Southern Brazil, whereas six groups of propolis were collected from Northeastern Brazil. Furthermore, one group of propolis was found in Southeastern Brazil. It was found that the variety of propolis is depending on plant ecology. Although rarely exceptional propolis was found in spite of geographic locations, these propolis were not included in this study.
Physiological activities for 12 groups of propolis were also variables, depending on geographical location. This is due to fact that the compositions of propolis are depending on the compositions in plants. Mit Apitherapieprodukten wurden Kranke behandelt, die periodisch zwischen 5 und 10 Jahren untersucht wurden.
Authors presented results in conservative treatment of 54 cases of hip joint with aseptic necrosis of thigh bone. In 22 hips, excluding the typical conservative treatment, APE injections were given. However, in the remaining 32, different forms of relieve were used. The obtained results in the first group A confirm the purpose of enrichment in conservative treatment by adapting intra- articular injections of APE especially in advanced stages of necrosis III-IV period of illness and also in those whose parents did not express their consent on surgery in the early stage of the illness.
Previous studies from this laboratory have established that caffeic acid esters present in propolis, a natural resin produced by honey bees, are potent inhibitors of human colon adenocarcinoma cell growth, carcinogen-induced biochemical changes, and preneoplastic lesions in the rat colon. The present study was designed to investigate the chemopreventive action of dietary phenylethylmethylcaffeate PEMC on azoxymethane-induced colon carcinogenesis and to examine the modulating effect of PEMC on phosphatidylinositol-specific phospholipase C PI-PLC , phospholipase A2, lipoxygenase LOX , and cyclooxygenase activities in the colonic mucosa and tumor tissues in male F rats.
At 7 weeks of age, all animals except those in the vehicle normal saline -treated groups were given 2 weekly s. All groups were maintained on their respective dietary regimen until the termination of the experiment 52 weeks after the carcinogen treatment. Colonic tumors were evaluated histopathologically. The precise mechanism by which PEMC inhibits colon tumorigenesis remains to be elucidated. It is likely that the chemopreventive action may be related, at least in part, to the modulation of PI-PLC-dependent signal transduction and LOX- mediated arachidonic acid metabolism.
It has been established that the use of apitherapy pollen and propolis to treat patients with ischemic insults leads to deeper positive shifts in indices of the antioxidant system and brain blood supply. In the specialised literature, the fact that propolis is used to treat a wide range of skin diseases dermatitis, eczema, herpes, various wounds, etc.
Honey has also been used in traditional medicine to treat skin injuries. It was demonstrated that this treatment is highly effective against decubitus ulcers. Gilglio, Atibaia, SP, Brasil, telefone Email: franksawaya amhanet. Este trabalho tem dois objetivos: 1. The presence of 19 elements has been shown in the ethanol extracts of propolis EEP. Three fractions have been obtained by filtration through a structural gel that did not show an initial antibacterial activity when investigated separately.
Fractions 2 and 3 joined together have regained this activity. EEP solutions maintain their anitbacterial activity in acidic or neutral pH. Insensitivity of EEP solutions on temperature of 75 degrees C for 30 min has been found. Investigation of the sensitivity of Staphylococci isolated from pathological cases to ethanol extract of propolis EEP. No cross-resistance of the staphylococci to EEP and to any commonly used antibiotics was found.
The induction of resistance to EEP in laboratory strain of Staphylococcus aureus Oxford P can be achieved already after serial passages on nutrient media containing EEP. Culturing Staphylococcus resistant to EEP in an environment devoid of this compound caused a remission to sensitivity of the strain investigated. An increase of total cell number was shown in the cell culture in vitro under the influence of ethanol extract of propolis EEP. Addition of EEP to the nutrient medium of the cells caused a strong activation of mitoses.
Besides, distinctly intensified metabolism of these cells expressed by a strong activation of NADH2-reductase was also observed. Dressing of artificially formed losses of the cartilaginous tissue with the preparation containing ethanol extract of propolis EEP caused acceleration of regenerating processes in the lesioned cartilage.
EEP inserted into the joint is well tolerated. Under experimental conditions parenteral administration of ethanol extract of propolis EEP solutions to rabbits induced no synthesis of antibodies as investigated by means of ring precipitation, double diffusion gel precipitation and complement fixation test. The use of ethanol extract of propolis EEP on injured dental pulp results in the stimulation of regenerative processes. A reduction of disorders of the circulatory system is observed, inflammatory and degenerative processes are also reduced. Mice were given i.
Some of the animals were sacrificed immediately following the last injection and some within weeks after the last injections. Internal organ samples were taken and underwent a microscopic examination. Insignificant pathological changes in the liver were observed. These changes were transient and regressed within weeks after the application of EEP. Antitumoral effect of ethanolic extract of propolis EEP was demonstrated in mature mice-bearing Ehrlich carcinoma.
Survival rate after EEP treatment was compared to that of bleomycin, given alone or in combination every two days for 36 days and followed up for 14 additional days. It is concluded that while the in vivo activity of bleomycin is reduced in the presence of cytochrome-C-reductase inhibitors like some of the EEP components are , the antitumoral property of EEP in the tumored animal model studied is significant and lasting. The activity of propolis and its combinations with antibiotics against antibiotic resistant strains of Staphylococcus aureus was studied.
It was found that staphylococcae strains resistant to benzylpenicillin, tetracycline and erythromycin were mainly sensitive to propolis. It is concluded that there was synergism in the effect of propolis and antibiotics with respect to antibiotic resistant strains of Staphylococcus aureus.
The materials used comprised of the following: i purified propolis and propolis saturated with antiinflammatory trace metal elements and ii propolis saturated with trace metal elements and poplar bud ointment saturated with trace metal elements also. Both methods of application using all the three preparations significantly improved symptoms. The preparations saturated with metallic ions were more effective.
The mild effect of the placebo treatment is explained by the treatment procedure itself. Side effects were not observed. Email: avallejos agr. Solutions of the ethanol extract of propolis EEP have shown a lethal effect on Trichomonas vaginalis in vitro.
Similar lethal action was exhibited by EEP after a h contact with Toxoplasma gondii. Purpose: To investigate the antibacterial properties of propolis and honey against oral bacteria in vitro and in vivo. Materials and Methods: In vitro study: The antibacterial effects of propolis and honey on oral streptococci were determined using the broth method. Clinical study: The short-term antibacterial effect of propolis solution and honey on salivary total bacteria and Streptococcus mutans was tested in 10 volunteers.
Results: Propolis demonstrated an antibacterial effect both in vitro on isolated oral streptococci and in the clinical study on salivary bacterial counts. Honey induced bacteria growth at low concentrations, while at high concentrations honey had an inhibitory effect on bacterial growth in vitro. Salivary counts of total bacteria and Streptococcus mutans were lower for 1 hour after application of honey.
The antibacterial effect of the honey tested may be attributed to its osmolarity effect. Artificially induced bone tissue losses after the application of ethanol extract of propolis EEP showed an accelerated rate of ossification. The osteogenetic process was just about half as long as in the control group. Zwischen und wurden im Apitherapiezentrum von Kamianna 4.
Ethanolic and aqueous extracts of the natural compound propolis indicate substantial antiinflammatory functions as well as antibiotic activities in vitro and in vivo. The exact mode of physiological or biochemical mechanisms responsible for the medical effects, however, is all but clear. The standardization on the basis of quantitative determination of prominent components of these extracts have been substituted recently by simple biochemical model reactions including photodynamic properties.
In this communication we report on the inhibitory activity of an aqueous extract of propolis on the enzyme dihydrofolate reductase. This activity may at least partially be due to the content of caffeic acid, as revealed by HPLC chromatography and comparative activity tests of representative ingredients of the propolis extract. The active component of the honeybee hive product propolis, caffeic acid phenethyl ester CAPE , induces a selective growth suppressive and toxic effect toward cloned rat embryo fibroblast cells transformed by adenovirus type 5 Ad5 or the Ad5 E1A transforming gene versus untransformed cloned rat embryo fibroblast cells Z-z.
Su et al. For this investigation we used somatic cell hybrids and 5- azacytidine-treated Ad5-transformed rat embryo cells that display different stages of expression of the transformed phenotype. This series of cell lines has permitted us to determine whether expression of the transformed state and the stage of transformation progression regulates CAPE sensitivity. Evidence is presented indicating that sensitivity to CAPE is directly determined by the state of expression of the transformed progression phenotype, as opposed to simply the expression of the Ad5 E1A and E1B transforming genes.
These results provide further evidence that CAPE may represent a unique compound that can specifically target progressed transformed cells for growth suppression and toxicity. An understanding of the mechanism underlying this selective effect of CAPE could result in the identification of important biochemical pathways mediating cellular transformation and progression of the transformed state.
The active component of the folk medicine propolis, caffeic acid phenethyl ester CAPE , displays selective toxicity toward cloned rat embryo fibroblast CREF cells transformed by a spectrum of diverse acting oncogenes. Identification of the mode of action of CAPE should provide useful information for possible applications of this compound for cancer therapy. The present study uses a series of oncogene transformed, oncogene-reverted and CAPE-resistant oncogene transformed CREF cells to investigate the mechanism underlying the increased sensitivity of transformed cells to CAPE. These findings indicate that expression of the transformed phenotype by rodent cells evokes sensitivity to CAPE induced toxicity through apoptosis.
The acquisition of CAPE sensitivity in rodent cells is independent of the mode of action of the oncogenic agent. CAPE may prove useful as an antiproliferative agent in cancer cells transformed by mechanistically diverse acting oncogenes. Spanish abstract: Eficacia del propoleos como remedio tricomonicida, en ensayos in vivo e in vitro. Para el cultivo, emplearon un liofilizado de cultivo de T. El cultivo fue repartido entre 10 probetas. Al cabo de 10 dias de terapia local, el examen de control puso de manifiesto la ausencia completa de T. Caffeic acid phenethyl ester, an active component of propolis extract, inhibits 5-lipoxygenase in the micromolar concentration range.
The inhibition is of an uncompetitive type, i. Caffeic acid phenethyl ester also exhibits antioxidant properties. The observed therapeutic effects were shortening of the disease duration.
The regression of symptoms occurred in the first day of the therapy and the complete recovery followed in 1 day in 5 patients, in 2 day in 16, and in 3 day in 3. The placebo group has his full recovery in mean 4. In the therapeutic group the symptoms lasted 2. Ruta Provincial E-mail: ebedas inta. Experience with the treatment of patients with panaritium, abscesses, phlegmons, infectious wounds have shown that propolis is an expedient remedy in additional to the routine treatment. They have shown the stimulating, antiinflammatory and anti-microbial action of propolis.
Dieses produkt wurde bei der Behandlung von Paradontopathien verwendet. Die Untersuchungen erfolgten mit Hunden, vor allem der Beagle-Rasse. The target of action of several anti-HIV substances including alkaloids O-demethyl-buchenavianine, papaverine , polysaccharides acemannan , lignans intheriotherins, schisantherin , phenolics gossypol, lignins, catechol.
Standardization and antioxidative properties of ethanolic and aqueous derivatives , in Zeitschrift fur Naturforschung. Abstract Ethanolic extracts of Propolis are used as antiinflammatory and wound healing drugs since ancient times. One of these activity tests bases on the inhibition of peroxidase-catalyzed oxidation of indole acetic acid indicating the presence of a defined mixture of monophenolic and diphenolic compounds.
Other tests diaphorase-catalyzed reductions and xanthine oxidase-catalyzed oxidations demonstrate significant radical scavenging properties. Water-soluble extracts of propolis exhibit higher antioxidative and inhibitory activities as compared to the ethanolic extract. Photodynamic activities , in Zeitschrift fur Naturforschung. Section C. Journal of Biosciences , Nov- Dec. In order to standardize the principle activities of prominent components of these extracts, simple biochemical tests have been introduced in the preceding paper.
These activity tests prove the high antioxidative and inhibitory capacities of aqueous and ethanolic extracts of propolis in vitro. In the present communication we report on experiments documenting photodynamic quenching properties of these extracts. Using riboflavin, rose bengal or hematoporphyrin as photoactivators and ketomethylthiobutyric acid or crocin as indicators, the protective functions of propolis preparations can be demonstrated.
The results indicate that the aqueous extracts are more active than the corresponding ethanolic preparation. However, the biochemical mechanisms of propolis effects in wound healing and inflammatory processes are not yet fully understood. Therefore the effects of different ethanolic and aqueous extracts on leukocytes and some of their most important enzymes namely myeloperoxidase, NADPH oxidase and lipoxygenase were investigated.
Only high concentrations of propolis extracts inhibited these enzymatic activities but especially the water-soluble derivatives showed stimulatory effect on the activity of commercially available human myeloperoxidase. Leukocytic myeloperoxidase and NADPH oxidase activities were clearly inhibited by propolis extracts probably indirectly due to their excellent radical scavenging properties.
Effects of caffeic acid and chlorogenic acid on mutagenicity were studied using the Salmonella typhimurium system. These compounds had inhibitory effects on the mutagenicity of Trp-P-1 and Glu-P Caffeic acid completely eliminated the mutagenicity induced by activated Glu-P- 2. Some compounds analogous to caffeic acid, such as cinnamic acid, coumaric acid, and ferulic acid, also significantly decreased the mutagenicity of Glu-P Veterinary Institute of Kazan, Leningrad, USSR, International Beekeeping Congress, Bologna, Roma, International Beekeeping Congress, Bucharest, Romania, Apimondia Publishing House.
Romanian edition, pages , IV-Th. New York, U. Les produits apicoles et leurs technologie. On the composition and utilisation of bee products and preparations in nutrition and therapeutics with regard to their biological value. Apimondia Publishing House , Bucharest, Romania. English edition, , pp. Propolis pag. Wellingborough, Northhampshire: Thorsons Publ. ISBN IX-Th edition. Medical Publishing House , Bucharest, Romania, Apimondia Congress, Beijing, China, September , Apimondia Publishing House, Bucharest, Romania, pp.
Lund , Denmark — The natural product Propolis. A way to health. Mentor Verlag. Abreu, J. Abusuev, S. Apimondia Congress, Antwerp, Belgium. Brother Adam U. Northern Bee Books. Hebden Bridge West Yorkshire, U. Library of Congress Catalog Card No. Aga, H. Akopyan, Z. Alexandrova L. Alexandrov, I.
Alexandru, V. BS 30 : Alphandery, E. Bernemann Ed. Anastasiu, R. Experience of the O. Apetroaiei, N. Artemov, M. Artomasova A. Arvouet — Grand,A. Asis, M. Composicion y uso de la miel, cera, polen, jalea real, propoleo y veneno de las abejas Spanish. Centro de Informacion y Documentacion Agropecuario.
Meaningfulness of apitherapeutic approaches using the example of primary dysmenorrhea
La Habana. Aspoy, E. Ludguni, Babin, R. Baikowa, R. Balalykina V. Kasan Publishing Co. Bankova, V. Bankova V. Barre, R. CA 36 : Basnet, P. Bellegrandi, S. Bell, W. Apimondia Congress, Nagoya, Japan, , p. Belomyttseva L. Berngard, K. CA 86 : u. Bianchi, E. Billiard, R. Lille, Le Biogt. Binder, W.
ISBN pages Bodea, C. Editura Academiei , Bucuresti, Romania. Bogdan, I. Bohrisch, P. Boiteux, R. Bojda, Z. Bojor,Ovidiu, Iliesiu,N. Bojor, Ovidiu; Iliesiu, N. Bolshakova V. BA 47 Bolshakova I. Bonimond, J. Bousquet, J. Pages: Brangi, G.
Propolisul, Bibliografie A-Z | Apiterapie
Brehm, Reinhold [xii] Germany — Propolis, ein Dauerbrenner! Brown, Ron U. Bunney, M. Bunta, S. Burdock, G. CA 18 : Canolle, L. Carre, P. Cattorini, P. Ceakarova R. Apimondia Congress, Nagoya, Japan non-presented paper. Chauvin, R. II: Les produits de la ruche. Masson et Cie , Paris, France. Librarie Hachette edit. Cheng, Paul C. Cherniak, N. Chlorazak, T. IM : Christensen, H. For Teknisk biochemi.
Christov, R. Ciani, A. Ciuchi, M. Ciuchi, V. Izolare si identificare a acidului cafeic traducere. Documentare apicola , vol. Cizmarik, J. Isolation and identification of 4-hydroxymethoxy-cinnamic acid ferulic acid from propolis, in Journal of Apicultural Research , 12 1 , p. Coatmeur, Y.
Biochemical, Cellular and Medicinal Properties. Alan R.
Liss : New York. Colita, D. Constantinescu, M. Biologia, allevamento, prodotti. Corsi, M. Crane Eva U. London : International Bee Research Association. Heinemann , London, U. Crisan Iuliana, Petica,M. Cristea, A. Cuculescu, N. Curylo, J. Danilov, L. Dano, P. Davydov, A. Deblock-Bostyn, G. Derevici Adelina, Popescu,A.
Derevici Adelina, Popescu,Al. Derevici Adelina, Ioanitiu,R. Derevici Adelina, Zalmanovici,R. Derevici Adelina et al. Derevici Adelina , , , Romania — Contribution to the study of propolis. Detoma, P. Dieterich, K. CA 2 : CA 5 : Digrak, M. Di Maggio, G. Dimov, V. Priroda , Bratislava, Czechoslovakia. Dobrowolski, J. Verlag Maloine , 2. Maloine Editeur S. Liliana Bretotean.
Doroshenko, P. Draganova L. Drogovoz, S. Dumitrescu Ana, Iliesiu, N. The antioxidant action on human fibroblast cultures abstract , in Roum. Dustmann, H. Vitalkraft und Heilwirkung der Bienenprodukten. Echigo, T. Egger, K. El — Banby, M. Al-Ahram Centre for Translation and Publishing , ed2, pages. El-Ghazaly, M. Erwin C. Esanu, V. Et Folk. Fearnley, James U. Fearnley, J. Natural Healing from the Hive.
Souvenir Press , London, UK. Feiks, Franz Klemens Austria — Topical application of propolis tincture in the treatment of herpes zoster , in the Third International Symposium on Apitherapy, Portoroz, Yugoslavia, , p. Feodorov, V. Ferguson, A. Ferreres, F. Feueressl, R.
Punta del Este. Rio Cuarto Cordoba. V Jornada de produccion animal de la Plata. Tel-Aviv, Israel, mayo. Fierro Morales, Walter Uruguay — Clinical evaluation of a new hypoallergic formula of propolis in dressings in Bee Products: properties, applications and apitherapy. New York and London, Cap. Filipic, B. Focht, J. Bogans forlag. Fontana, Jose Domingos et al.
Franco, F. Frariere, A. Frenkel, M. Augsburg: Midena Verlag. Propolis pages: 67, 84, f. Frolov, V. Cuba Marzo Gabor, M. Akademiai Kiado , Budapest. Gabor, C. Gafar, M. Letter Portuguese , in Soc. Susanne Reinerth, Die harzartigeSubstanz Propolis istdas Allheilmittel der Bienen. Auchdie Bienen selbst werden mithilfevon Propolis gesund gehalten.
Propolis ist eine harzige Substanz, die von Bienen hergestellt wird, um damit den Bienenstock abzudichten. Die Abdichtung des Roland Lackner, Bienen desinfizieren mit dem harzigen Propolis auch Bienen- kittharz genannt das Innere und den Eingangsbereich ihres Bienenstocks. Markus Minoggio, Propolis is a resinous substance produced by honey bees when they mix their saliva and beeswax with nectar or sap from plant sources such as flowers and Global Propolis Industry Market research report spreads across pages, profiling 21 key companies and is supported with tables and figures.
This is a El propolis es un producto preciado en el siglo XXI a medio camimo entre los medicamentos y los alimentos. Es lo que se llama un 'medicalimento', apunta Kovandan gelen mucize propolis nedir? Hautpflege mit Propolis gegen Alters-und Pigmentflecken. Als Kittharz der Bienen dient es der Infektionsabwehr aufgrund fungizider und Eine noch neue Sparte in der Apitherapie ist der Propolis -Bienenduft-Verdampfer, der zur Verbesserung der Raumhygiene eingesetzt wird.
Propolis ist das von